|
Esther Wangui; sample preparation at NMK |
The
Barcode of Wildlife Project Kenya (BoWPK) will hold its Academics and Informatics training
Workshop between 22nd to 30th
April 2014. The objective of the workshop is to test the cost-effectiveness,
scalability, and real-world impact of a DNA-based technology on the enforcement
of species protection laws and treaties.
During this period, animal samples from Kenya Wildlife Service
(KWS) Forensic and Genetics Laboratory, and plants species from National Museums of Kenya (NMK)
have been assembled to be used to isolate DNA for sequencing and subsequent
preparation of DNA barcodes.
The animal
samples were isolated from healthy live animals collected from different parts
of the country. Samples for DNA isolation were obtained from animals free from
any observable infections.
In the meantime,
Consortium of Barcode of Life (CBOL) has prepared short introductory videos for
each consultation and module introducing the training plan, BARCODE data
standard and the extension of the standard that will be used in Barcode of
Wildlife Project.
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Sealed samples for the workshop |
Informatics
training workshop has been distributed into different modules; What is a Field
Information Management System (FIMS) and FIMS spreadsheet?, Introduction and
installation of Geneious, General Introduction to the Geneious software, Validating
specimen data & uploading to FIMS, Importing specimen data from FIMS to
LIMS, creating Extraction Plates, Creating PCR Plates, Creating Cycle
Sequencing Plates, Cherry Picking, Editing Sequences, Sequence Analysis, and Uploading
final data to GenBank.
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Sealed samples for the workshop |
Barcode of
wildlife project is a collaborative project between National Museums of Kenya
(NMK), Kenya wildlife Service (KWS), Consortium of Barcode of Life and Google. The
project is designed as a proof-of-concept for ‘DNA barcoding’ as a system that
would be used in developing countries by
border inspectors, park rangers and other regulatory officials to detect
illegal trafficking in protected species.
The workshop will be held at both the laboratories facilities at the Biosciences eastern and central Africa-International Livestock Research Institute (BecA-ILRI) Hub, 22-26 April for Academics Training, and the International Centre of Insect Physiology and Ecology (ICIPE), an international scientific research institute, 28-30 April, for Informatics Training
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John Kariuki, KWS; sample sealing |
|
Dr Peris Kamau, NMK, during sample preparation |
|
NMK Research Scientists Esther Wangui and Brenda Nyaboke cross check accuracy of collected samples. |
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